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论文题目 Molecular cytogenetic characterization of a new wheat–rye 4R chromosome translocation line resistant to powdery mildew
作  者 Diaoguo An(安调过), Qi Zheng, Yilin Zhou, Pengtao Ma(马朋涛), Lihui Li, Bin Li, Qiaoling Luo(罗巧玲), Hongxing Xu(许红星), Zhenling Lv, Yunfeng Xu(许云峰);
发表年度 2013
刊物名称 Chromosome Research
卷、期、页码 Volume 21; Issue 4; pp 419-432
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论文摘要 Rye is an important and valuable gene resource for wheat improvement. However, due to extensive growing of cultivars with disease resistance genes from short arm of rye chromosome 1R and coevolution of pathogen virulence and host resistance, these cultivars successively lost resistance to pathogens. Identification and deployment of new resistance gene sources in rye are, therefore, of especial importance and urgency. A new wheat–rye line, designated as WR41-1, was produced through distant hybridization and chromosome engineering protocols between common wheat cultivar Xiaoyan 6 and rye cultivar German White. It was proved to be a new wheat–rye T4BL·4RL and T7AS·4RS translocation line using sequential genomic in situ hybridization (GISH), multicolor fluorescence in situ hybridization (mc-FISH), and expressed sequence tag-simple sequence repeat (EST-SSR) marker analysis. WR41-1 showed high levels of resistance to powdery mildew (Blumeria graminis f. sp. triticiBgt) pathogens prevalent in China at the adult growth stage and 13 of 23 Bgtisolates tested at the seedling stage. According to its resistant pattern to 23 different Bgt isolates, WR41-1 may possess new gene(s) for resistance to powdery mildew, which differed from previously identified and known powdery mildew genes from rye (Pm7Pm8Pm17, and Pm20). In addition, WR41-1 was cytologically stable, had a desirable fertility, and is expected to be useful in wheat improvement.
全文链接 http://dx.doi.org/10.1007/s10577-013-9366-8
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